COVID-19 Testing Pipeline: Lesson Learned
نویسندگان
چکیده
The transmission of severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) in Indonesia is seen to be uncontrollably increasing that urges the government leverage capacity for disease detections. Real-time polymerase chain reaction (RT-PCR), rapid test and computed tomography (CT) scan are most common methods determine if one has been infected regardless whether or not symptoms such Corona Virus Disease 2019 (COVID-19) surface. Among these three, RT-PCR considered gold standard qualitative quantitative assessment SARS CoV-2 detection. present paper aims at elaborating framework Roche's machine employed specifically detection performed by Genetics which deemed efficient relatively quicker than other kits. detected Cov-2 with RNA amplification curve equals 10 copies below cut off value Crossing point (Cp) positive control. Also elucidated implementations EAV LightCycler® 96 System through analysis time, amounts individual required sample, as well reagents, can accordingly reduced.
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ژورنال
عنوان ژورنال: IOP conference series
سال: 2021
ISSN: ['1757-899X', '1757-8981']
DOI: https://doi.org/10.1088/1755-1315/794/1/012118